Elite hybrid mass spectrometer creating new possibilities in research and discovery part 2 introducing the orbitrap elite. We know you, or someone you know, worked very hard to get your orbitrap based mass spectrometer in your lab and even harder preparing your precious samples. Product specifications schematic of the ltq orbitrap velos. This instrument combines a thermo scientific orbitrap mass analyzer and velos pro ion trap technology to deliver high resolution, speed, sensitivity, and flexibility. Ltq orbitrap velos with etd thermo fisher scientific applications. The thermo scientific ltq orbitrap velos combines the proven mass accuracy and ultra high resolution of the orbitrap mass analyzer, with the increased s ensitivity and improved cycle time of the ltq velos 7khkljk mass accuracy of the ltq orbitrap velos increases the. Instrument schematic of the orbitrap elite mass spectrometer. Dartthermo ltq orbitrap interface manual for thermo ltq orbitrap velos, ltq orbitrap xl, ltq ion trap, exactive high performance, and all other instruments that utilize the ion max source version 20100814 ionsense inc. The thermo ltq orbitrap velos mass spectrometer is a hybrid mass spectrometer incorporating the ltq velos dual cell linear trap and the orbitrap analyzer. Prosightpc analyze topdown and middledown proteomics data. Ltq velos, nanospray flex, nanoviper, orbitrap elite, orbitrap velos pro, proteome discoverer, and. Achieving peak performance from your velos pro, orbitrap.
Orbitrap systems connect to liquid chromatography equipment to enhance separation of unknown compounds and enable highthroughput workflows. Benchmark platform for confident structural elucidation. Metabolite analysis untargeted metabolomics and metabolite structural elucidation. Pierce ltq velos esi positive ion calibration solution.
Thermo scientific ltq velos dualpressure linear ion trap 2 the thermo scienti. Most of these instruments have atmospheric pressure ion sources though an intermediatepressure maldi source can also be used maldi ltq orbitrap. Schematic of the ltq orbitrap velos ms instrument with openi. Download scientific diagram schematic presentation of orbitrap velos pro etd. Exactive generated 1083% more protein groups and 11109% more peptides than the ltq. How much orbitrap q exactive plus costs compared to. The velos pro ion trap contributes enhanced ion optics that increase sensitivity and reliability, a greater dynamic range for better precursor detection, and the power of msn identification. Characterization of breast cancer interstitial fluids by. While there are two positive solutions, there is only one negative solution. Thermo scientific ltq velos pro mass spectrometer system. Ltq orbitrap xl hybrid ion trap orbitrap mass spectrometer support troubleshooting find valuable information.
Schematic of the orbitrap velos pro mass spectrometer. The thermo finnigan ltq is a stateoftheart, segmented linear ion trap mass spectrometer. Thermo scientific ltq orbitrap velos hy b rid ft mass. Tight integration with the ion injection process enables the highresolution, mass accuracy, and sensitivity. All information in this guide concerning the orbitrap velos pro mass. Velos pro dualpressure linear ion trap mass spectrometer support troubleshooting orbitrap elite hybrid ion trap orbitrap mass spectrometer support ltq orbitrap xl hybrid ion trap orbitrap mass spectrometer support. We want this system to be accompanied by the best analysis software as well. Thermo scientific proteome discoverer software for comprehensive workflowdriven. Methods using exclusively cidfragmentation when acquiring msms spectra consisted of an orbitrap full ms scan followed by up to 20 ltq msms experiments top20 on the most abundant ions detected in the full ms scan. The high mass accuracy of the ltq orbitrap velos increases the speed and con. Schematic of the ltq orbitrap xl mass spectrometer with an hcd collision cell. For the ltq velos, velos pro, ltq orbitrap velos, orbitrap velos pro, and orbitrap elite. Thermo xcalibur qual browser workstation software facilitates processing of data.
A dual pressure linear ion trap orbitrap instrument with very high. But, skyline can also export srm method files for the thermoscientific ltq. Pdf a dual pressure linear ion trap orbitrap instrument. About this guide this ltq orbitrap discovery hardware manual contains a description of the modes of operation and principle hardware components of your ltq orbitrap. Ltq orbitrap velos is a member of the family of ltq mass spectrometer ms hybrid instruments. Efa is a simple loan to your business that allows you to buy the equipment or software you need. Thermo fisher scientific orbitrap velos pro hardware manual pn 1288290, revision a i read this first welcome to the thermo scientific orbitrap velos pro system. Editors, technical documentation thermo electron bremen gmbh advanced mass spectrometry hannakunathstr. Ltq orbitrap velos is a hybrid mass spectrometer incorporating the ltq velos dual cell linear trap and the orbitrap analyzer.
The orbitrap velos pro mass spectrometer is a member of the family of ltq mass spectrometer ms detectors. Product specifications thermo scientific orbitrap elite. Methods using exclusively cidfragmentation when acquiring msms spectra consisted of an orbitrap full ms scan followed by up to 20 ltq msms experiments top20 on. Thermo scientific velos pro ion trap and orbitrap elite. Building on the industryleading thermo scientific orbitrap platform, the thermo scientific orbitrap velos pro hybrid ion trap orbitrap mass spectrometer features dualpressure linear ion trap technology that delivers increased sensitivity and dynamic range with ultimate robustness.
Figure 5 shows the schematic view of etd enabled thermo ltq velos pro. Wheels at the bottom side of the instrument facilitate positioning the ltq orbitrap xl at the intended place in the laboratory. All of these instruments provide a high mass accuracy ltq orbitrap elite suitable for prm. Analyze proteomics and metabolomics samples rapidly and with confidence using the thermo scientific orbitrap velos pro hybrid ion trap orbitrap mass spectrometer. These appear throughout the manual, where applicable. Welcome to the thermo scientific, ltq orbitrap discovery system. The orbitrap velos pro is a member of the family of ltq mass spectrometer ms hybrid instruments. The velos pro was custom designed for both qualitative and quantitative applications in the laboratory. The ltq orbitrap velos ms was used in the datadependent mode. About this guide this orbitrap velos pro getting started manual provides information on how to set up, calibrate, and tune the orbitrap velos pro mass spectrometer. Qexactive outperformed ltq orbitrap velos hcd in terms of number of peptide and protein groups, due to faster scan rate and higher resolution. An optional electron transfer dissociation etd module and.
Building on a tradition of innovation, the ltq velos provides the analyst with signi. Unique dualpressure linear ion trap increases sensitivity, scan speed and mass resolution. A, the stacked ring ion guide slens increases the ion flux from the electrospray ion source into the. A collision cell for performing higher energy cid experiments. From the microsoft windows taskbar, choose start programs. Ltq orbitrap series preinstallation requirements planet orbitrap.
The ltq orbitrap xl features an hcd collision cell to provide additional. Product specifications mass spectrometry combining the next generation thermo scientific velos pro ion trap with the new highfield orbitrap technology, the thermo scientific orbitrap elite hybrid ms provides the ultimate level of versatility and performance for your research. Evolution of orbitrap mass spectrometry instrumentation. More product data calibration solutions for thermo scientific mass spectrometers. In hybrid orbitrap ms elite, velos pro, velos mass isolation is through ion trap. Thermo scientific proteome discoverer software for.
Reference manual for the ltq velos, velos pro, ltq orbitrap velos. The ltq orbitrap xl is a member of the family of ltq mass spectrometer ms detectors. The orbitrap mass analyzer is the first highperformance mass analyzer that employs trapping of ions in electrostatic fields. Technical information on the orbitrap technology platform including the orbitrap elite, orbitrap velos pro, ltq orbitrap xl, q exactive, exactive plus, exactive, ltq orbitrap discovery, ltq orbitrap velos, and the ltq orbitrap. You can access pdf files of the documents listed above from the data system computer. Schematic view of the ltq orbitrap velos the ltq orbitrap velos has the following measuring specifications. Relative quantitation of tmtlabeled proteomes focus on. Schematic of the orbitrap velos pro hybrid mass spectrometer with etd. All information in this guide concerning the ltq orbitrap velos also applies to the ltq orbitrap velos etd system where the etd module is physically coupled to the back of the ltq orbitrap velos. Resources for optimal performance of your orbitrap velos pro mass spectrometer.
The ltq orbitrap velos and qexactive mass spectrometers were compared for shotgun proteomic analysis of 1 to 1,000 ng raw 264. Thermo scientific offers a wide portfolio of orbitrap based ms systems, including systems combined with ion traps and quadrupole mass filters, to address all your analysis needs. Orbitrap elite, ltq orbitrap velos, isobaric tagging, quantitative precision. Schematic of the ltq orbitrap velos ms instrument with three new. Analyze demanding proteomics, metabolomics, lipidomics and metabolism samples in greater depth using the thermo scientific orbitrap elite hybrid ion trap orbitrap mass spectrometer. Procedures in chapters 14 can be pe rformed from the xcalibur tune plus window.
Relative quantitation of tmtlabeled proteomes focus on sensitivityand precision. A schematic representation of the gradient profile is reported in table 3 supporting information. The thermo scientific orbitrap elite mass spectrometer combines a dual linear ion trap velospro with a highfield orbitrap mass analyzer. Ltq orbitrap velos getting started concordia university. Schematic of the ltq orbitrap velos ms instrument with three new hardware implementations. Ltq orbitrap xl hybrid ion traporbitrap mass spectrometer. The ltq orbitrap is a high performance lcms and msn system, combining rapid ltq ion trap data acquisition with high mass accuracy orbitrap mass analysis. B, the dual linear ion trap design enables efficient trapping and activation in the highpressure cell left and fast scanning and detection in the low pressure cell. Note thermo fisher scientific no longer ships the ltq velos and lxq mass spectrometers. Using the industryleading, highresolution, accuratemass thermo scientific orbitrap technology with biopharma finder software, the most accurate and confident ptm profiles and identifications can be achieved more quickly than ever before.
Most of this information is also pertinent to the ltq velos and ltq orbitrap velos mass spectrometers. Principle of operation schematic of the ltq orbitrap xl. An ion trap instrument like the ltq may not have the sensitivity of a triple quadrupole, but you can still use one for targeted proteomics, and you can use srm on the ltq as a quality control measure for. About this guide this ltq orbitrap xl etd hardware manual contains a description of the modes of operation and principle hardware components of your ltq orbitrap xl etd instrument. Welcome to the thermo scientific, ltq orbitrap xl system. The thermo scientific ltq orbitrap velos combines the proven mass accuracy and ultra high resolution of the orbitrap mass analyzer, with the increased s ensitivity and improved cycle time of the ltq velos 7khkljk mass accuracy of the ltq orbitrap velos increases the speed and confidence of identification in complex. Orbitrap achieving peak performance on the orbitrap portion of the orbitrap velos pro and orbitrap elite hybrid instruments. Refurbished thermo orbitrap systems gentechscientific. Relative quantitation of tmtlabeled proteomes focus on sensitivity and precision r. Protein analysis bottomup proteomics, topdown proteomics, post translational modification ptm analysis and relative quantitation.
We discuss the evolution of orbitrap mass spectrometry ms from its birth in the late 1990s to its current role as one of the most prominent techniques for ms. The achieving peak instrument performance reference manual. Jan 15, 20 the ltq orbitrap velos and qexactive mass spectrometers were compared for shotgun proteomic analysis of 1 to 1,000 ng raw 264. A, the stacked ring ion guide slens increases the ion flux from the electrospray ion source into the instrument by a factor 510.
This instrument combines a highfield thermo scientific orbitrap mass analyzer and thermo scientific velos pro ion tra. Orbitrap velos pro hardware manual thermo fisher scientific. The ltq velos esi positive ion calibration solution can be used to calibrate the ltq velos velos pro, the ltq orbitrap velos orbitrap velos pro, the orbitrap elite, and the qexactive mass spectrometer instruments. In addition to the above described, every instrument has specific. How much orbitrap q exactive plus costs compared to orbitrap exactive plus. Ltq orbitrap velos is the next generation mass spectrometers, thanks to its significantly increased sensitivity and scan speed. Results and discussion the elution profile for the 11 compounds is provided in. Ltq orbitrap velos labelfree protein quantitation protein identification biological annotation experimental sample preparation mice kindly provided to inserm by niams, nih, bethesda, md were sacrificed and muscle tissues were collected, snapfrozen in liquid nitrogen for 30 seconds, and stored at 80 c. Service your orbitrap from circuit boards to complete bench repair we can return your systems to optimal performance. This ltq orbitrap velos getting started manual is intended for all personnel that. Microsoft windows 7 professional 32bit and 64bitthermo foundation 2. Ltq orbitrap velos is a member of the family of ltq mass spectrometer ms detectors. Ltq orbitrap xl getting started, orbitrap velos pro getting started, or orbitrap elite.
Ions can be selected in the linear ion trap and fragmented either in the ion trap cid or in the hcd collision cell. Who uses this guide this ltq orbitrap xl getting started manual is intended for all personnel that needs to operate the ltq orbitrap xl, especially the key operator. Cid, pqd, etd, and now traphcd fragmentation increases flexibility for structural elucidation fast, sensitive ion trap for the most demanding applications generation ii ion optics improve robustness and reduce downtime. You would not be able to open the older methods anymore since its not. Ltq orbitrap velos hardware manual brown university. Velos pro dualpressure linear ion trap mass spectrometer.
Clientserver application that uses workflows to process and report mass spectrometry data. An orbitrap analyzer for fourier transformation b ased analysis. Schematic presentation of orbitrap velos pro etd mass. Zabrouskov 1thermo fisher scientific, san jose, ca. Hardware manual, orbitrap velos pro hardware manual, or orbitrap. The fragmentation data show that the sensitivities of the cid and hcd spectra are comparable on the orbitrap velos pro mass spectrometer. Welcome to the thermo scientific orbitrap velos pro system. About this guide this ltq orbitrap xl getting started manual provides you with information on how to set up, calibrate, and tune the ltq orbitrap xl.
Extended warranty extend your peace of mind with our extended warranty. The thermo ltq orbitrap velos mass spectrometer is a hybrid mass spectrometer incorporating the. About this guide this ltq orbitrap velos getting started manual provides information on how to set up, calibrate, and tune the ltq orbitrap velos. Orbitrap elite hybrid ion traporbitrap mass spectrometer. Equipped with an electrospray ionization esi source, the ltq boasts improved capacity and trapping efficiency, higher scan speed and increased sensitivity. To accomplish this on ltq orbitrap, ltq orbitrap xl, and ltq orbitrap discovery mass spectrometers, a toggle for analysis of highmolecularweight molecules is accessible from the instrument control window tune page in instru ment control software version 2. Welcome to the thermo scientific ltq orbitrap velos system. Lipid analysis untargeted lipidomics, shotgun lipidomics. The ltq orbitrap xl etd is a member of the family of ltq mass spectrometer ms hybrid instruments.
Thermo finnigan ltq msms ion max esi source gentech. Sciencebased website focusing on orbitrap mass spectrometers with workflows for proteomics, metabolomics, lipidomics, pharmaceuticals, biopharmaceuticals, clinical, drugs of abuse, sports doping, environmental, and food safety for life science and analytical researchers. Orbitrap mass analyzer and detection of the transient. Comparison of ltqorbitrap velos and qexactive for proteomic. Qexactive outperformed ltq orbitrap velos hcd in terms of number of peptide and protein groups, due. Orbitrap velos pro getting started thermo fisher scientific. Mass spectrometry analysis on the ltq orbitrap velos. Schematic of the ltq orbitrap velos ms instrument with. Dartthermo ltq orbitrap interface manual for thermo ltq orbitrap velos, ltq orbitrap xl, ltq ion trap, exactive high performance, and all other instruments that utilize the ion max source version 20120423 ionsense inc. Welcome to the thermo scientific ltq orbitrap xl etd system.
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